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Anti-platelet effect of ginkgolide a from Ginkgo biloba

Journal of the Korean Society for Applied Biological Chemistry volume 57, pages 221–228 (2014)Cite this article

Abstract

Effects of ginkgolide A (GA) from Ginkgo biloba leaves in collagen (10 μg/mL)-stimulated platelet aggregation were investigated. Zymographic analysis confirmed that pro-matrix metalloproteinase-9 (MMP-9) (92 kDa) was activated by GA to form an activated MMP-9 (86-kDa) on gelatinolytic activities. GA concentration-dependently inhibited platelet aggregation, intracellular Ca2+ mobilization, and thromboxane A2 (TXA2) formation by inhibiting the cyclooxygenase-1 (COX-1) activity in collagen-stimulated platelets. In addition, GA increased the formation of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP), which have an anti-platelet function in both resting and collagen-stimulated platelets. On the other hand, GA did not prolong prothrombin time (PT) and activated partial thromboplastin time (aPTT) associated with the extrinsic and intrinsic coagulation pathways on human plasma, respectively. Therefore, we suggest that the inhibitory effect of GA on platelet aggregation might involve the following pathway. GA may increase the MMP-9 activity and intracellular cAMP and cGMP production, inhibit intracellular Ca2+ mobilization, and decrease TXA2 production by down-regulating the COX-1, thereby leading to inhibition of platelet aggregation without cytotoxicity. These results strongly indicate that GA is a potent inhibitor of collagen-stimulated platelet aggregation. It may play an important role as a negative regulator during platelet activation.

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Author notes

  1. Jin-Hyeob Ryu

    Present address: Department of Microbiology and Immunology, Institute of Medical Science, Tokyo University, Minato-ku, Tokyo, 108-8639, Japan

Authors and Affiliations

  1. Department of Biomedical Laboratory Science, College of Medical Science, Konyang University, 685, Gasuwon-dong, Seo-gu, Daejeon, 302-718, Republic of Korea

    Jin-Hyeob Ryu, Ju-Ye Ro & Hyun-Jeong Cho

  2. Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering and Regional Research Center, Inje University, 607, Obang-dong, Gimhae, Gyungnam, 621-749, Republic of Korea

    Hwa-Jin Park

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  1. Jin-Hyeob Ryu
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Correspondence to Hyun-Jeong Cho.

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Jin-Hyeob Ryu and Ju-Ye Ro contributed equally to this work.

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Ryu, JH., Ro, JY., Park, HJ. et al. Anti-platelet effect of ginkgolide a from Ginkgo biloba . J Korean Soc Appl Biol Chem 57, 221–228 (2014). https://doi.org/10.1007/s13765-013-4275-2

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