Fig. 2From: Anti-invasive effect of β-myrcene, a component of the essential oil from Pinus koraiensis cones, in metastatic MDA-MB-231 human breast cancer cellsEffect of major EOPC constituents on MMP-9 promoter activity. A MMP-9 promoter activity assay. MDA-MB-231 cells were transiently transfected with pMMP9(−925/+13)_Luc. At 48 h post-transfection, the cells were either left untreated or treated with 10 ng/mL TNFα in the absence or presence of EOPC components (100 μM). B Cytotoxicity assay. MDA-MB-231 cells were treated with TNFα or EOPC components as in (A) for 24 h. The data shown represent the mean ± SD of three independent experiments performed in triplicate. **p < 0.01Back to article page