Table 1 Expression of the amylase-encoding gene in L. plantarum
Expression vector | Gene | Extracellular fraction | Intracellular fraction | Secretion efficiency | ||
|---|---|---|---|---|---|---|
Activity (U/L) | Protein (mg/L) | Activity (U/L) | Protein (mg/L) | % | ||
pSIP409 | AmyL7 | 2120 ± 31.8 | 117.7 ± 1.1 | 221.0 ± 1.1 | 422.0 ± 18.2 | 91.6 ± 0.12 |
AmyL9 | 0.0 | 158.5 ± 7.4 | 91.9 ± 4.8 | 464.4 ± 8.6 | – | |
pSIP609 | AmyL7.1 | 1984.1 ± 103.6 | 80.8 ± 6.4 | 218.2 ± 0.8 | 364.2 ± 6.5 | 90.1 ± 0.61 |
AmyL9.1 | 0.0 | 148.6 ± 3.2 | 180.3 ± 9.4 | 537.9 ± 24.7 | – | |
L. plantarum WCFS1 | 0.0 | 126.95 ± 8.2 | 0.0 | 391.3 ± 10.4 | – | |
L. plantarum TGL02 | 0.0 | 105.3 ± 10.7 | 0.0 | 376.8 ± 15.6 | – | |