Skip to main content
Applied Biological Chemistry
Fig. 3 | Applied Biological Chemistry

Fig. 3

From: Establishment of a loop-mediated isothermal amplification (LAMP) assay for the detection of phytoplasma-associated cassava witches’ broom disease

Fig. 3

Optimization of HNB as an indicator of positive LAMP reactions. (A) Effect of different HNB concentrations on its color reaction. Tube 1, 2: 120 µM HNB; Tube 3, 4: 100 µM HNB, Tube 5, 6: 80 µM HNB. Tube 1, 3, 5: Positive; tube: 2, 4, 6: Negative control. (B) Limit of detection of a positive reaction by HNB color change; tube (−): no DNA template; 0–8: 100–108 dilutions of the 100 ng initial phytoplasma DNA in each LAMP reaction

Back to article page