Table 1 LAMP primers for the detection of phytoplasma 16S rDNA and cassava actin-coding sequence
Primers | Primer’s length (bp) | Sequences | Product’s length (bp) |
|---|---|---|---|
1-FIP | 49 | 5′-GGT GTT CCT CCA TAT ATT TAC GCA TCT AGA GTA AGA TAG AGG CAA GTG-3′ | 223 |
1-BIP | 39 | 5′-CTG ACG CTG AGG CAC GAA AGA GTA CTC ATC GTT TAC GGC-3′ | |
1-F3 | 23 | 5′-CAT TGT GAT GCT ATA AAA ACT GT-3′ | |
1-B3 | 20 | 5′-CAA CAC TGG TTT TAC CCA AC -3′ | |
2-FIP | 42 | 5′- TGC ACC ACC TGT GCA ACT GAT AAG GTC TTG ACA TGC TTC TGC-3′ | 221 |
2-BIP | 41 | 5′-TGG GTT AAG TCC CGC AAC GAG CTT GCT AAA GTC CCC ACC AT-3′ | |
2-F3 | 20 | 5′- AGG TAC CCG AAA AAC CTC ACC-3′ | |
2-B3 | 19 | 5′- TCC CCA CCT TCC TCC AAT T-3′ | |
Actin FIP | 43 | 5′-GCT TCT CCT TCA TGT CAC GGA CTG ATG AAG ATC CTC ACT GAG A-3′ | 270 |
Actin BIP | 44 | 5′-TGA ACA GGA ACT TGA GAC TGC CCA TCA GGA AGC TCA TAG TTC TT-3′ | |
Actin F3 | 18 | 5′-GCT CTT CCA CAT GCC ATT-3′ | |
Actin B3 | 20 | 5′-CTT CTG GAC AAC GGA ATC TT-3′ |