Establishment of a loop-mediated isothermal amplification (LAMP) assay for the detection of phytoplasma-associated cassava witches’ broom disease

Applied Biological Chemistry

Table 1 LAMP primers for the detection of phytoplasma 16S rDNA and cassava actin-coding sequence

Primers	Primer’s length (bp)	Sequences	Product’s length (bp)
1-FIP	49	5′-GGT GTT CCT CCA TAT ATT TAC GCA TCT AGA GTA AGA TAG AGG CAA GTG-3′	223
1-BIP	39	5′-CTG ACG CTG AGG CAC GAA AGA GTA CTC ATC GTT TAC GGC-3′
1-F3	23	5′-CAT TGT GAT GCT ATA AAA ACT GT-3′
1-B3	20	5′-CAA CAC TGG TTT TAC CCA AC -3′
2-FIP	42	5′- TGC ACC ACC TGT GCA ACT GAT AAG GTC TTG ACA TGC TTC TGC-3′	221
2-BIP	41	5′-TGG GTT AAG TCC CGC AAC GAG CTT GCT AAA GTC CCC ACC AT-3′
2-F3	20	5′- AGG TAC CCG AAA AAC CTC ACC-3′
2-B3	19	5′- TCC CCA CCT TCC TCC AAT T-3′
Actin FIP	43	5′-GCT TCT CCT TCA TGT CAC GGA CTG ATG AAG ATC CTC ACT GAG A-3′	270
Actin BIP	44	5′-TGA ACA GGA ACT TGA GAC TGC CCA TCA GGA AGC TCA TAG TTC TT-3′
Actin F3	18	5′-GCT CTT CCA CAT GCC ATT-3′
Actin B3	20	5′-CTT CTG GAC AAC GGA ATC TT-3′