Skip to main content

Table 1 LAMP primers for the detection of phytoplasma 16S rDNA and cassava actin-coding sequence

From: Establishment of a loop-mediated isothermal amplification (LAMP) assay for the detection of phytoplasma-associated cassava witches’ broom disease

Primers Primer’s length (bp) Sequences Product’s length (bp)
1-FIP 49 5′-GGT GTT CCT CCA TAT ATT TAC GCA TCT AGA GTA AGA TAG AGG CAA GTG-3′ 223
1-BIP 39 5′-CTG ACG CTG AGG CAC GAA AGA GTA CTC ATC GTT TAC GGC-3′
1-F3 23 5′-CAT TGT GAT GCT ATA AAA ACT GT-3′
1-B3 20 5′-CAA CAC TGG TTT TAC CCA AC -3′
2-FIP 42 5′- TGC ACC ACC TGT GCA ACT GAT AAG GTC TTG ACA TGC TTC TGC-3′ 221
2-BIP 41 5′-TGG GTT AAG TCC CGC AAC GAG CTT GCT AAA GTC CCC ACC AT-3′
2-F3 20 5′- AGG TAC CCG AAA AAC CTC ACC-3′
2-B3 19 5′- TCC CCA CCT TCC TCC AAT T-3′
Actin FIP 43 5′-GCT TCT CCT TCA TGT CAC GGA CTG ATG AAG ATC CTC ACT GAG A-3′ 270
Actin BIP 44 5′-TGA ACA GGA ACT TGA GAC TGC CCA TCA GGA AGC TCA TAG TTC TT-3′
Actin F3 18 5′-GCT CTT CCA CAT GCC ATT-3′
Actin B3 20 5′-CTT CTG GAC AAC GGA ATC TT-3′