Establishment of a loop-mediated isothermal amplification (LAMP) assay for the detection of phytoplasma-associated cassava witches’ broom disease

Applied Biological Chemistry

Table 2 Primer sequences of nested PCR

	Primers	Sequences
Round 1	P1A	ACGCTGGCGGCGCGCCTTAATAC
Round 1	P7A	CCTTCATCGGCTCTTAGTGC
Round 2	R16F2n	GAAACGACTGCTAAGACTGG
Round 2	R16R2	TGACGGGCGGTGTGTACAAACCCCG
Round 3	R16(I)F1	TAAAAGACCTAGCAATAGG
Round 3	R16(I)R1	CAATCCGAACTGAGAATGT