Round 1 | 94 °C | 94 °C | 55 °C | 72 °C | 72 °C | 15 °C |
4 min | 1 min | 2 min | 3 min | 7 min | 10 min | |
1 cycle | 38 cycles | 1 cycle | 1 cycle | |||
Round 2 | 94 °C | 94 °C | 50 °C | 72 °C | 72 °C | 10 °C |
1 min 30 s | 30 s | 50 s | 1 min 20 s | 10 min | 10 min | |
1 cycle | 30 cycles | 1 cycle | 1 cycle | |||
Round 3 | 94 °C | 94 °C | 50 °C | 72 °C | 72 °C | 10 °C |
1 min 30 s | 1 min | 2 min | 3 min | 10 min | 15 min | |
1 cycle | 34 cycles | 1 cycle | 1 cycle |