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Applied Biological Chemistry
Fig. 2 | Applied Biological Chemistry

Fig. 2

From: α-Pinene inhibits tumor invasion through downregulation of nuclear factor (NF)-κB-regulated matrix metalloproteinase-9 gene expression in MDA-MB-231 human breast cancer cells

Fig. 2

Effect of α-pinene on the inhibition of TNFα-induced MMP-9 mRNA expression. (A) RT-PCR analysis. MDA-MB-231 cells were treated with 10 ng/mL TNFα in the absence or presence of α-pinene for 18 h. GAPDH mRNA was used as an internal control. (B) MDA-MB-231 cells were treated with 10 ng/mL TNFα in the absence or presence of α-pinene for 18 h. Relative fold changes in the mRNA levels between untreated control and TNFα- or TNFα plus α-pinene-treated cells were measured by quantitative real-time PCR. The relative fold changes were normalized to GAPDH mRNA in the same sample. The data shown represent the mean ± SD of three independent experiments performed in triplicate (B). ** P < 0.01 versus TNFα-only treatment (n = 9)

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