Skip to main content
Fig. 3 | Applied Biological Chemistry

Fig. 3

From: α-Pinene inhibits tumor invasion through downregulation of nuclear factor (NF)-κB-regulated matrix metalloproteinase-9 gene expression in MDA-MB-231 human breast cancer cells

Fig. 3

Effect of α-pinene on the inhibition of TNFα-induced MMP-9 promoter activity. MDA-MB-231 cells were transfected with 50 ng pRL-null vector and 0.2 μg of pMMP9(–925/+13)_Luc. Then, 48-h post-transfection, cells were treated with 50 µM α-pinene for 8 h, and their luciferase activities were determined. Values for firefly luciferase were normalized to those for Renilla luciferase. Data represent the mean ± SD of three independent experiments, performed in triplicate. The data shown represent the mean ± SD of three independent experiments performed in triplicate. ** P < 0.01 versus TNFα-only treatment (n = 9)

Back to article page