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Applied Biological Chemistry
Fig. 4 | Applied Biological Chemistry

Fig. 4

From: α-Pinene inhibits tumor invasion through downregulation of nuclear factor (NF)-κB-regulated matrix metalloproteinase-9 gene expression in MDA-MB-231 human breast cancer cells

Fig. 4

Effect of α-pinene on the inhibition of TNFα-induced NF-κB activity. (A) Immunoblot analysis. MDA-MB-231 cells were treated with 10 ng/mL TNFα in the absence or presence of 50 μM α-pinene. Whole-cell lysates were prepared, and immunoblotting was performed using the phospho-specific antibody against IKK (Ser176/180), IκBα (Ser32) or RelA/p65 (Ser536). The anti-GAPDH antibody was used as an internal control. (B) NF-κB-dependent transcriptional activity assay. MDA-MB-231 cells were transfected with 5 × NFκB_Luc plasmid, along with 50 ng pRL-null. At 48-h post-transfection, the cells were either untreated or treated with 10 ng/mL TNFα in the absence or presence of 50 μM α-pinene. The data shown represent the mean ± SD of three independent experiments performed in triplicate. **P < 0.01 versus TNFα-only treatment (n = 9)

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