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Applied Biological Chemistry
Fig. 5 | Applied Biological Chemistry

Fig. 5

From: α-Pinene inhibits tumor invasion through downregulation of nuclear factor (NF)-κB-regulated matrix metalloproteinase-9 gene expression in MDA-MB-231 human breast cancer cells

Fig. 5

Effect of α-pinene on the inhibition of TNFα-induced NF-κB phosphorylation. MDA-MB-231 cells were either treated with DMSO (vehicle) or treated with 10 ng/mL TNFα in the absence and presence of 50 μM α-pinene for 30 min. Primary antibodies specific to α-tubulin and phospho-p65/RelA (Ser536) were incubated for 2 h, followed by incubation with Alexa Fluor 488-conjugated (green) and Alexa Fluor 555-conjugated (red) secondary antibodies for 30 min. Nuclear DNA (blue) was stained with 1 μg/mL Hoechst for 10 min. Stained cells were analyzed by EVOSf1fluorescence microscope. Immunofluorescence microscopic analysis using Alexa Fluor 488-conjugated (green signal) or Alexa Fluor 555-conjugated (red signal) secondary antibodies. Nuclear DNA was stained with 1 μg/mL Hoechst 33258 (blue signal)

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