Fig. 6

SK-HEP-1 cells viability by BNCE treatment and light irradiation. Cells were treated with BNCE (50 and 100 μg/ml) for 4 h. Irradiated with light for 10 min or kept in the dark, followed by incubation for 6 h. Cells were stained with Muse™ Annexin V and Dead cell kit for 20 min and analyzed using a Muse™ Cell Analyzer. Results show a dose-dependent increase in the number of apoptotic U937 cells compared to dark conditions. (A), (B) Scatter plots showing distribution of Annexin V and 7-AAD staining (UL: dead cells, UR: late apoptotic/dead cells, LL: live cells, and LR: early apoptotic cells). The (C), (D) represent % of cells and the summary mean percentages ± SD of apoptosis (early and late apoptosis) of three independent experiments in dark and light. Statistical analysis was analyzed using one-way ANOVA