Fig. 2

Effects of omega-7 on cell viability, collagen regeneration, and elasticity factor expression against oxidative stress in H₂O₂-treated HaCaT cells. (A) Viability of HaCaT cells after treatment with increasing concentrations of H₂O₂ (0.1–1 mM) for 24 h. (B) HaCaT cells after treatment with 1 mM H₂O₂ for 24 h after pretreatment with omega-7 (1–20 μg/mL) for 1 h. (D–E) Whole cell lysates were subjected to Western blot analysis to evaluate MMP-1, PCOL1, and elastin expression. *P < 0.05, **P < 0.01 versus CON, ^#P < 0.05, ^##P < 0.01 versus H₂O₂