Table 1 Primer sequences were used in this work
Gene | Sequence (5′–3′) | |
|---|---|---|
Primers for qRT-PCR analysis | ||
miR-124 | Forward | TACTATAAGGCACGCGGTGAATGC |
Reverse | GCGAGCACAGAATTAATACGAC | |
U6 | Forward | CGCTTCGGCAGCACATATACTA |
Reverse | CGCTTCACGAATTTGCGTGTCA | |
Bim | Forward | TCATCGCGGTATTCGGTTC |
Reverse | GAAGGTTGCTTTGCCATTTG | |
β-Actin | Forward | GGCATCCTCACCCTGAAGTA |
Reverse | GGGTGTTGAAGGTCTAAA | |
Primers for luciferase activity analysis | ||
Bim-WT | Forward | CGGGGTACCGCGGTTCTCTTGTGGAGGGG |
Reverse | CCCAAGCTTAGAAGGGGAAACGGCAGACA | |
Bim-mut | Forward 1 | CGGGGTACCGCGGTTCTCTTGTGGAGGGG |
Reverse 1 | GTAATCTCTAAAAATAAAGCTTTTTAAGTTAGCTGGC | |
Forward 2 | CGGGGTACCGCCAGCTAACTTAAAAAGCTTTATTTTTAGAGATTAC | |
Reverse 2 | CCCAAGCTTCTCTTTCTTGGGTCTCCCTGGGC | |
Forward 3 | CGGGGTACCGCCCAGGGAGACCCAAGAAAGAG | |
Reverse 3 | CACATAGGGAGAGGTTGTCAAAACCTGCAGTAATTTCC | |
Forward 4 | CGGGGTACCGGAAACTTACTGCAGGTTTTGACAACCTCTCCCTATGTG | |
Reverse 4 | CCCAAGCTTAGAAGGGGAAACGGCAGACA | |