Development of DNA-based species-specific real-time PCR markers for four berry fruits and their application in commercial berry fruit foods

An, Jun; Moon, Jun-Cheol; Kim, Ju Hee; Kim, Geum Sol; Jang, Cheol Seong

doi:10.1186/s13765-019-0413-9

Applied Biological Chemistry

Table 4 The amplified C_t values of 18 commercial berry food products by qPCR using the developed primer sets

From: Development of DNA-based species-specific real-time PCR markers for four berry fruits and their application in commercial berry fruit foods

Sample numbers	18S rRNA	Aronia		Blackberry		Cranberry		Strawberry
Sample numbers	18S rRNA	(matK)	(trnL-F)	(trnL-F)	(rbcL)	(matK)	(rbcL)	(matK)	(trnL-F)
1	14.56 ± 0.06	18.42 ± 0.18	19.39 ± 0.17	37.14 ± 1.46	35.62 ± 0.49	31.52 ± 1.12	31.97 ± 0.13	35.84 ± 0.43	36.05 ± 0.34
2	14.54 ± 0.07	18.63 ± 0.07	19.07 ± 0.01	38.79 ± 0.66	36.12 ± 0.67	40.78 ± 1.92	38.02 ± 0.63	37.67 ± 0.95	35.45 ± 0.92
3	12.83 ± 0.13	18.69 ± 0.3	19.72 ± 0.13	39.77 ± 0.83	35.17 ± 0.48	40.5 ± 2.87	39.18 ± 1.16	35.17 ± 0.41	35.96 ± 0.68
4	13.66 ± 0.06	19.29 ± 0.14	19.78 ± 0.17	33.16 ± 0.35	32.14 ± 0.21	32.88 ± 3.13	30.71 ± 0.18	34.43 ± 0.19	34.57 ± 0.41
5	15.49 ± 0.07	22.77 ± 0.28	22.37 ± 0.18	35.36 ± 1.25	35.82 ± 0.29	ND	36.8 ± 1.18	34.73 ± 0.12	39.85 ± 0.9
6	13.92 ± 0.11	34.93 ± 0.25	ND	17.97 ± 0.16	17.82 ± 0.08	35.45 ± 2.3	35.5 ± 0.51	33.29 ± 0.18	35.29 ± 0.59
7	13.96 ± 0.16	40.12 ± 2.73	ND	16.33 ± 0.19	16.18 ± 0.16	38.94 ± 5.62	38.14 ± 1.72	33.2 ± 0.61	33.84 ± 0.22
8	14.08 ± 0.15	40.18 ± 3.36	ND	16.5 ± 0.05	16.28 ± 0.07	34.89 ± 2.4	34.46 ± 0.5	34.48 ± 0.23	35.37 ± 0.36
9	14.07 ± 0.1	40.75 ± 2.56	ND	16.59 ± 0.18	16.1 ± 0.1	40.58 ± 3.65	39 ± 1.39	32.71 ± 0.08	34.35 ± 0.26
10	15.55 ± 0.07	34.8 ± 0.42	38.18 ± 0.99	22.38 ± 0.12	24.3 ± 0.08	40.06 ± 0.94	38.37 ± 0.46	36.67 ± 4.07	34 ± 1.684
11	15.62 ± 0.16	ND	39.48 ± 2.96	37.37 ± 0.35	40.23 ± 6.33	19.23 ± 0.01	22.22 ± 0.13	42.76 ± 0.85	38.29 ± 0.7
12	15.92 ± 0.18	ND	40.84 ± 0.43	31.35 ± 0.18	32.38 ± 0.35	19.37 ± 0.08	23.35 ± 0.24	41.47 ± 0.38	33.87 ± 0.51
13	15.68 ± 0.15	ND	35.99 ± 1.28	31.42 ± 0.15	33.96 ± 0.43	18.17 ± 0.01	20.3 ± 0.11	40.33 ± 0.22	33.4 ± 0.2
14	14.32 ± 0.05	39.75 ± 1.96	ND	34.63 ± 0.34	30.45 ± 0.17	38.37 ± 3.48	40.42 ± 0.11	17.04 ± 0.18	18.2 ± 0.04
15	17.15 ± 0.13	37.39 ± 2.75	ND	35.62 ± 0.76	38.59 ± 2.75	36.73 ± 1.36	38.75 ± 1.53	20.37 ± 0.07	21.03 ± 0.08
16	13.6 ± 0.06	36.85 ± 0.4	40.89 ± 1.65	36.3 ± 0.03	35.23 ± 1.33	37.72 ± 0.34	41.32 ± 3.38	17.55 ± 0.03	18.81 ± 0.13
17	13.59 ± 0.14	35.54 ± 0.63	38.11 ± 1.54	39.35 ± 0.63	40.1 ± 0.64	37.49 ± 2.06	42.9 ± 1.88	16.22 ± 0.07	18.02 ± 0.12
18	15.92 ± 0.05	34.04 ± 0.65	37.92 ± 1.18	30.17 ± 2.98	34.13 ± 0.6	38.4 ± 0.96	37.4 ± 0.46	22.97 ± 0.21	21.01 ± 0.15

qRT-PCRs were performed with three technical replicates
Numbers in italic indicate C_t values of species—specific primers in each sample

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