Comparison of headspace–SPME and SPME-Arrow–GC–MS methods for the determination of volatile compounds in Korean salt–fermented fish sauce

Applied Biological Chemistry

Table 1 Comparison of SPME and SPME-Arrow extraction efficiencies of volatile compounds in fish sauce

Peak No.	Volatile compounds	RT (min)	RI	Relative concentration (mg/L)
				SPME-Arrow		SPME
				Average	SD	Average	SD
Ketones (1)
1	2-Butanone	6.81	912	0.074^b	0.004	0.125^a	0.014
Aldehydes (3)
2	2-Methyl-butanal	7.06	925	0.101^a	0.006	0.102^a	0.02
3	3-Methyl-butanal	7.13	928	0.178^a	0.014	0.169^a	0.018
4	Benzaldehyde	33.04	1634	2.109^a	0.03	1.171^b	0.212
Alcohols (5)
5	Ethanol	7.49	946	0.536^a	0.06	0.265^b	0.04
6	1-Penten-3-ol	15.38	1205	0.066^b	0.006	0.158^a	0.021
7	3-Methyl-1-butanol	17.58	1258	0.122^a	0.01	nd^b	nd
8	2-Furanmethanol	39.56	1807	0.884^a	0.036	nd^b	nd
9	Phenylethyl alcohol	50.7	2137	1.288^a	0.029	nd^b	nd
Pyrazines (6)
10	2,5-Dimethylpyrazine	23.25	1394	0.581^a	0.023	0.463^a	0.083
11	2,6-Dimethylpyrazine	23.56	1401	0.691^a	0.027	0.596^a	0.088
12	2-Ethyl-6-methyl-pyrazine	26.38	1469	0.315^a	0.007	0.208^b	0.017
13	Trimethylpyrazine	27.37	1493	0.421^a	0.011	0.241^b	0.046
14	2-Ethyl-5-methylpyrazine	27.65	1500	0.05^a	0.001	0.038^b	0.005
15	3-Ethyl-2,5-dimethylpyrazine	28.78	1528	0.02^a	0.001	0.01^b	0.001
Acids (7)
16	Acetic acid	29.33	1541	2.953^a	0.726	1.328^a	0.082
17	Propanoic acid	33.72	1652	0.611^b	0.075	0.916^a	0.092
18	2-Methylpropanoic acid	35.19	1689	0.794^b	0.102	1.116^a	0.076
19	Butanoic acid	37.95	1764	2.684^b	0.158	5.296^a	0.487
20	3-Methylbutanoic acid	39.87	1816	6.253^b	0.263	8.970^a	0.904
21	2-Methylpentanoic acid	44.4	1947	0.242^b	0.012	0.484^a	0.046
22	4-Methylpentanoic acid	45.95	1993	0.387^b	0.035	0.612^a	0.073

RT retention time, RI retention index—used for identification by GC–MS (DB-WAX column), Relative concentrations of volatile compounds are expressed as equivalents of the internal standard (2 mg/L of 4-methyl-2-pentanol) for SPME-Arrow and SPME–GC/MS, nd not detected
Data represent mean ± SD (n = 3). Different letters (a and b) represent significant differences (P < 0.05) using ANOVA and Duncan's multiple range test.