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Applied Biological Chemistry

Table 2 Relative quantification of GC‒MS-based metabolites in ‘Kwangan’ WT soybean and drought-tolerant transgenic soybean ‘ABF3’ measured after 9 weeks of moderate-drought treatment

From: Global metabolite profiling based on GC–MS and LC–MS/MS analyses in ABF3-overexpressing soybean with enhanced drought tolerance

Component

Kwangan

ABF3

t-value

Degrees of freedom

p value

Leucine

3.89 ± 0.79

5.46 ± 1.41

− 2.38

10

0.039

Asparagine

1.49 ± 0.55

3.75 ± 1.58

− 3.30

10

0.008

Valine

10.4 ± 3.01

16.3 ± 4.69

− 2.61

10

0.026

Lysine

0.29 ± 0.18

0.66 ± 0.21

− 3.11

9

0.013

Serine

1.58 ± 0.52

3.00 ± 1.03

− 3.02

10

0.013

Isoleucine

5.91 ± 1.21

8.76 ± 2.14

− 2.83

10

0.018

beta-Alanine

0.66 ± 0.35

1.51 ± 0.49

− 3.49

10

0.006

Glycine

0.46 ± 0.06

0.79 ± 0.23

− 3.35

10

0.007

Threonine

0.62 ± 0.17

1.11 ± 0.24

− 4.10

10

0.002

4-Keto glucose

0.06 ± 0.01

0.04 ± 0.01

3.08

10

0.012

Pyroglutamic acid

0.91 ± 0.17

1.68 ± 0.40

− 4.30

10

0.002

Threonic acid

0.40 ± 0.06

0.29 ± 0.04

3.76

10

0.004

Phenylalanine

3.54 ± 0.90

6.51 ± 1.85

− 3.53

10

0.005

d-Pinitol

106.3 ± 9.33

93.5 ± 3.12

2.95

6

0.026

3-Hydroxy hexadioic acid

0.22 ± 0.03

0.39 ± 0.07

− 5.32

10

< 0.001

Inositol

14.3 ± 1.40

19.9 ± 3.71

− 3.46

10

0.006

Tyrosine

4.91 ± 0.93

8.50 ± 1.43

− 5.16

10

< 0.001

  1. Relative amounts were determined by peak area ratio of analyte to internal standard. Values are means (n = 6) ± standard deviations. p-values are from Student’s t-tests
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