Table 3 Relative quantification of LC‒MS/MS-based metabolites in ‘Kwangan’ WT soybean and drought-tolerant transgenic soybean ‘ABF3’ measured after 9 weeks of moderate-drought treatment
Component | Kwangan | ABF3 | t-value | Degrees of freedom | p-value |
|---|---|---|---|---|---|
DG(14:0/18:0/0:0) | 5.44 ± 0.03 | 5.31 ± 0.06 | 4.96 | 10 | 0.001 |
Trigonelline | 5.64 ± 0.08 | 5.77 ± 0.07 | − 3.18 | 10 | 0.010 |
PI(20:0/20:3(8Z,11Z,14Z)) | 5.14 ± 0.09 | 5.01 ± 0.10 | 2.43 | 10 | 0.035 |
PI(20:3(5Z,8Z,11Z)/18:0) | 5.58 ± 0.09 | 5.40 ± 0.05 | 4.04 | 9 | 0.003 |
beta-d-Glucopyranosyl-11-hydroxyjasmonic acid | 5.92 ± 0.61 | 6.62 ± 0.28 | − 2.57 | 10 | 0.028 |
PI(18:1(11Z)/18:1(9Z)) | 4.44 ± 0.46 | 3.97 ± 0.23 | 2.25 | 10 | 0.048 |
PI(18:1(9Z)/20:4(8Z,11Z,14Z,17Z)) | 4.44 ± 046 | 3.97 ± 0.23 | 2.25 | 10 | 0.048 |
Enol-phenylpyruvate | 4.52 ± 0.11 | 4.78 ± 0.07 | − 4.67 | 10 | 0.001 |
Rubraflavone d | 4.04 ± 0.14 | 3.70 ± 0.34 | 2.28 | 10 | 0.046 |
Captopril-cysteine disulfide | 5.04 ± 0.09 | 4.76 ± 0.10 | 5.03 | 10 | 0.001 |
Gluten exorphin B5 | 4.85 ± 0.19 | 3.91 ± 0.62 | 3.26 | 9 | 0.010 |
Capsianoside VI | 5.61 ± 0.04 | 5.22 ± 0.32 | 2.84 | 6 | 0.029 |
Rheidin C | 4.54 ± 0.31 | 3.59 ± 0.14 | 4.09 | 6 | 0.006 |
N-(1-Deoxy-1-fructosyl)tyrosine | 5.03 ± 0.14 | 5.20 ± 0.10 | − 2.48 | 10 | 0.032 |
3-Methyl-3-butenyl apiosyl-(1- > 6)-glucoside | 3.77 ± 0.80 | 4.71 ± 0.16 | − 2.88 | 8 | 0.020 |
Benzyl O-[arabinofuranosyl-(1- > 6)-glucoside] | 3.58 ± 0.67 | 4.42 ± 0.16 | − 3.02 | 8 | 0.017 |
Veranisatin C | 5.25 ± 0.07 | 5.12 ± 0.05 | 4.07 | 10 | 0.002 |
N’-Hydroxyneosaxitoxin | 4.21 ± 0.38 | 3.53 ± 0.34 | 3.11 | 9 | 0.012 |
Dihydrozeatin-O-glucoside | 3.35 ± 0.38 | 4.25 ± 0.39 | − 3.62 | 8 | 0.007 |
Nequinate | 3.35 ± 0.38 | 4.25 ± 0.39 | − 3.62 | 8 | 0.007 |
Brassica napus non-fluorescent chlorophyll catabolite 3 | 8.09 ± 0.87 | 4.41 ± 1.69 | 4.33 | 8 | 0.002 |
5-Aminoimidazole ribonucleotide | 4.89 ± 0.42 | 5.35 ± 0.24 | − 2.32 | 10 | 0.043 |
Aromadendrin | 4.41 ± 0.28 | 3.86 ± 0.52 | 2.32 | 10 | 0.043 |
9-Deazainosine | 4.28 ± 0.27 | 3.79 ± 0.44 | 2.30 | 10 | 0.044 |
4-Methylthio-Alpha-d-Mannose | 5.17 ± 0.07 | 5.00 ± 0.04 | 5.54 | 10 | < 0.001 |
Malformin | 4.92 ± 0.33 | 4.32 ± 0.39 | 2.86 | 10 | 0.017 |
2,5-Pyridinedicarboxylic acid, dipropyl ester | 4.61 ± 0.30 | 5.05 ± 0.21 | − 2.93 | 10 | 0.015 |
d-Tyrosine | 5.45 ± 0.06 | 5.61 ± 0.04 | − 5.10 | 10 | < 0.001 |
Dihydroxyfumitremorgin C | 4.65 ± 0.11 | 4.88 ± 0.09 | − 4.17 | 10 | 0.002 |
Gingerglycolipid B | 4.73 ± 0.24 | 5.00 ± 0.12 | − 2.36 | 9 | 0.042 |