Fig. 1

T-DNA insertion mutation in the gcr1 gene in Arabidopsis thaliana and its expression pattern as analyzed by genotyping, and RT-PCR and qRT-PCR (a) diagram showing the position of T-DNA insertion based on the flanking sequences. Black line and white boxes represent the introns and exons, respectively. b Genotyping PCR of the T-DNA insertion knock-out line (gcr1). The primers used were as follows: P1, 5′-UTR region primer; P2, 3′-UTR region primer; LBb1, T-DNA left border primer. c RT-PCR analysis of the GCR1 transcript. d qRT-PCR validation of the mutant. The relative expression of the GCR1 transcript was calculated by the delta–delta Ct (ddCt) method and was converted to relative expression ratio (2^−ddCt). The expression of AtEF1α was used as the control. The primers used for the analyses are listed as in Additional file 1: Table S1