Fig. 2

The comparision of four types of beta-glucans in structure and effect on skin cells. a Viabilty of HaCaT and c fibroblast treated with beta-glucans derived from S. commune, black yeast, barely, and euglena. The cells (2 × 10⁴ cells/ml) were seeded and treated with 0–40 μg/ml beta-glucan for 24 h. Viability of cells was measured using the MTT solution (5 mg/ml). b Scratch wound healing assay with HaCaT treated as described in a after 24 h. HaCaT cells (7 × 10⁴ cells/well) were seeded on 48 well plate and a scratch was made on the cell monolayer by drawing a pipette tip across the well, and then treated with 0–40 μg/ml. Migration distance was measured using the ImageJ program. *P < 0.05 as compared to the control. d Western blotting for HaCaT cells treated with four types of beta-glucans. Cell lyastes were isolated using RIPA solution and protein concentration was measured by BCA assay. Phospho-FAK, E-cadherin, and slug expression were measured and their levels were normalized to GAPDH. The values indicate intensities of protein expression with respect to that of the loading control