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Fig. 3 | Applied Biological Chemistry

Fig. 3

From: The wound healing effect of four types of beta-glucan

Fig. 3

The stimulation effect of beta-glucan derived from S. commune on keratinocyte migration. a Viability of HaCaT and d fibroblast treated with beta-glucans derived from S. commune (0–400 μg/ml). The cells (2 × 104 cells/ml) were seeded and treated with 0–40 μg/ml for 24 h. Viability of cells was measured using the MTT solution (5 mg/ml). b Scratch wound healing assay with HaCaT treated as described in a after 24 h. HaCaT cells (7 × 104 cells/well) were seeded on a 48 well plate and a scratch was made on the cell monolayer by drawing a pipette tip across the well. Migration distance was measured using the ImageJ program. *P < 0.05 as compared to the control. c Transwell invasion assay with HaCaT cells treated with beta-glucans derived from S. commune. Invasive ability was measured by fixing with 4% of formaldehyde and staining with a 1% crystal violet solution. The photographs were acquired at × 4 magnification using a microscope. *P < 0.05 as compared to the control

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