Fig. 4

The action mechanisms of beta-glucan (S. commune) on keratinocyte and fibroblat. a–c Western blotting for HaCaT cells treated with the S. commune beta-glucan in a time-dependent manner. Cell lysates were isolated using RIPA solution and protein concentration was measured by BCA assay. The expression levels were normalized to GAPDH. The values indicate intensities of protein expression with respect to that of the loading control. d, e Western blotting for HaCaT cells treated with the S. commune beta-glucan in a time-dependent manner. Cell lysates were isolated using RIPA solution and protein concentration was measured by BCA assay. The expression levels were normalized to GAPDH. The values indicate intensities of protein expression with respect to that of the loading control