Evaluation of polyembryony for genetic resources and efficacy of simple sequence repeat markers for the identification of nucellar and zygotic embryo-derived individuals in citrus

Applied Biological Chemistry

Table 3 Characteristics of 17 polymorphic SSR markers developed in this study

Markers	SS	N_OBS	Availability	M_AF	N_A	GD	H_O	PIC
BM-CiSSR-012	101	101	1.00	0.42	7	0.75	0.62	0.72
BM-CiSSR-013	101	98	0.97	0.35	10	0.77	0.29	0.73
BM-CiSSR-32	101	101	1.00	0.49	14	0.72	0.67	0.70
BM-CiSSR-043	101	92	0.91	0.73	12	0.46	0.14	0.45
BM-CiSSR-073	101	101	1.00	0.37	13	0.77	0.84	0.75
BM-CiSSR-077	101	101	1.00	0.55	14	0.67	0.62	0.65
BM-CiSSR-082	101	101	1.00	0.48	9	0.71	0.40	0.68
BM-CiSSR-087	101	101	1.00	0.62	5	0.51	0.50	0.43
BM-CiSSR-093	101	101	1.00	0.63	5	0.53	0.62	0.47
BM-CiSSR-094	101	101	1.00	0.41	8	0.67	0.60	0.62
BM-CiSSR-100	101	101	1.00	0.42	7	0.68	0.85	0.62
BM-CiSSR-111	101	101	1.00	0.47	8	0.64	0.43	0.58
BM-CiSSR-115b	101	101	1.00	0.47	11	0.74	0.48	0.71
BM-CiSSR-137	101	100	0.99	0.34	16	0.80	0.84	0.78
BM-CiSSR-159	101	101	1.00	0.22	14	0.85	0.86	0.84
BM-CiSSR-162	101	99	0.98	0.22	16	0.84	0.58	0.82
BM-CiSSR-165	101	99	0.98	0.31	12	0.80	0.18	0.78
Mean	101	100	0.99	0.44	10.6	0.70	0.56	0.67

SS sample size, N_OBS number of observations, M_AF major allele frequency, N_A number of alleles, GD genetic diversity, which is often referred to as expected heterozygosity, H_O observed heterozygosity, PIC polymorphism information content