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Table 1 LC MS/MS conditions for PFCs analysis

From: Reduction of perfluorinated compound content in fish cake and swimming crab by different cooking methods

HPLC MS/MS
Equipment CD-C C18 column (3.0 μM particle diameter, 2.0 × 150 mm) Ionization mode ESI negative
API 4000 spectrometer Ion source gas 1 40 psi
Electrospray ionization (ESI) with negative mode Ion source gas 2 60 psi
Mobile phase A: 5 mM Ammonium acetate with 0.02% formic acid in water
B: Methanol
Curtain gas 25 psi
Injection volume 3 μL Ion spray voltage 4500 V
Flow rate 200 μL/min Collision gas 6 eV
Gradient   0 min 5 min 13 min 13.1 min 25 min Temperature 400 °C
A 70% 0% 0% 70% 70%   
B 30% 100% 100% 30% 30%