HPLC | MS/MS | |||||||
---|---|---|---|---|---|---|---|---|
Equipment | CD-C C18 column (3.0 μM particle diameter, 2.0 × 150 mm) | Ionization mode | ESI negative | |||||
API 4000 spectrometer | Ion source gas 1 | 40 psi | ||||||
Electrospray ionization (ESI) with negative mode | Ion source gas 2 | 60 psi | ||||||
Mobile phase | A: 5 mM Ammonium acetate with 0.02% formic acid in water B: Methanol | Curtain gas | 25 psi | |||||
Injection volume | 3 μL | Ion spray voltage | 4500 V | |||||
Flow rate | 200 μL/min | Collision gas | 6 eV | |||||
Gradient | 0 min | 5 min | 13 min | 13.1 min | 25 min | Temperature | 400 °C | |
A | 70% | 0% | 0% | 70% | 70% | |||
B | 30% | 100% | 100% | 30% | 30% |