Fig. 2From: Anti-inflammation activity of brazilin in TNF-α induced human psoriasis dermatitis skin modelBrazilin suppressed phosphorylation of IκB and Akt, not NF-κB, in TNF-a stimulated HaCaT cells. Cells were treated with each concentration of brazilin in the presence of TNF-a (20 ng/ml) for 2 h. Brazilin suppresses phosphorylation of IκB in TNF-α stimulated HaCaT cells in a concentration-dependent manner (a), but not NF-κB (c). The function of brazilin on inhibiting Akt phosphorylation in TNF-α stimulated HaCaT cells (e). Quantitative assay of p-IκB (b), p-NF-κB (d) and p-Akt (f) with image J program. Data are presented as mean ± S.D. of triplicate determinations. ##p < 0.01, compared to control, *p < 0.05, **p < 0.01 compared to TNF-α groupBack to article page