Simultaneous determination of various platycosides in Four Platycodon grandiflorum cultivars by UPLC-QTOF/MS

Lee, Dae Young; Choi, Bo-Ram; Lee, Jae Won; Um, Yurry; Yoon, Dahye; Kim, Hyoung-Geun; Lee, Young-Seob; Kim, Geum-Soog; Lee, Youn-Hyung; Baek, Nam-In

doi:10.1186/s13765-019-0457-x

Applied Biological Chemistry

Table 1 The list of various platycosides analyzed in Platycodi Radix (ESI−)

From: Simultaneous determination of various platycosides in Four Platycodon grandiflorum cultivars by UPLC-QTOF/MS

No.	RT (min)	Platycosides	Molecular formula	Expected neutral mass (Da)	Observed neutral mass (Da)	QTOF/MS (ESI−) m/z	Mass accuracy (ppm)	Adducts
1	2.59	platycoside L	C₄₂H₆₈O₁₇	844.4457	844.4449	843.4376	0.87	−H
2	3.8	Platy saponin A	C₄₂H₆₈O₁₆	828.4507	828.4484	827.4411	2.83	−H
3	3.9	Platycoside G1	C₆₄H₁₀₄O₃₄	1416.6409	1416.6399	1461.6381	0.66	+HCOO
4	4.07	Platycoside E	C₆₉H₁₁₂O₃₈	1548.6832	1548.6823	1593.6805	0.54	+HCOO
5	4.26	Platycoside D	C₆₉H₁₁₂O₃₇	1532.6882	1532.6853	1577.6835	1.84	+HCOO
6	4.35	Platycoside K	C₄₂H₆₈O₁₇	844.4457	844.4421	843.4348	4.22	−H
7	5.18	Deapioplatycodin D3	C₅₈H₉₄O₂₉	1254.5881	1254.5834	1299.5816	3.64	+HCOO
8	5.53	Platycodin D3	C₆₃H₁₀₂O₃₃	1386.6303	1386.6291	1431.6273	0.84	+HCOO
9	5.91	Platycoside H	C₅₈H₉₄O₂₈	1238.5932	1238.5936	1283.5918	0.34	+HCOO
10	6.34	Platycoside G3	C₆₃H₁₀₂O₃₂	1370.6354	1370.6409	1415.6391	3.85	+HCOO
11	8.3	Platycoside A	C₅₈H₉₄O₂₉	1254.5881	1254.5853	1299.5835	2.15	+HCOO
12	8.31	Platycoside F	C₄₇H₇₆O₂₀	960.493	960.4908	1005.489	2.19	+HCOO
13	8.64	Deapioplatycodin D	C₅₂H₈₄O₂₄	1092.5353	1092.5343	1137.5325	0.83	+HCOO
14	8.97	3′-O-Acetyl-polygalacin D	C₅₉H₉₄O₂₈	1250.5932	1250.5937	1295.5919	0.39	+HCOO
15	9.13	Platycoside C	C₅₄H₈₆O₂₅	1134.5458	1134.5461	1179.5443	0.23	+HCOO
16	9.23	Platycodin D2	C₆₃H₁₀₂O₃₃	1386.6303	1386.627	1431.6252	2.3	+HCOO
17	9.36	Platycodin D	C₅₇H₉₂O₂₈	1224.5775	1224.5793	1269.5775	1.44	+HCOO
18	9.63	Polygalacin D2	C₆₃H₁₀₂O₃₂	1370.6354	1370.6356	1415.634	0.15	+HCOO
19	9.66	3′-O-Acetyl-platycodin D2	C₆₅H₁₀₄O₃₄	1428.6409	1428.644	1427.6367	2.16	−H
20	9.78	Platyconic acid C	C₅₂H₈₂O₂₅	1106.5145	1106.5153	1105.508	0.7	−H
21	9.8	Platycodin C	C₅₉H₉₄O₂₉	1266.5881	1266.5899	1311.5881	1.42	+HCOO
22	10.01	Polygalacin D	C₅₇H₉₂O₂₇	1208.5826	1208.584	1253.5822	1.11	+HCOO
23	10.25	Platyconic acid D	C₅₄H₈₄O₂₆	1148.5251	1148.5261	1147.5188	0.91	−H
24	10.5	Platycodin J	C₅₈H₉₂O₃₀	1268.5673	1268.5684	1313.5666	0.84	+HCOO
25	10.56	Platyconic acid A lactone	C₅₇H₈₈O₂₈	1220.5462	1220.5476	1219.5403	1.15	−H
26	10.62	Platyconic acid A	C₅₇H₉₀O₂₉	1238.5568	1238.555	1237.5477	1.43	−H
27	10.79	2′-O-Acetyl-polygalacin D	C₅₉H₉₄O₂₈	1250.5932	1250.5946	1295.5928	1.08	+HCOO
28	11.02	Methyl-2-O-methylplatycogenate A	C₅₉H₉₄O₂₉	1266.5881	1266.5858	1265.5786	1.77	−H
29	11.25	Platyconic acid B	C₅₉H₉₂O₃₀	1280.5673	1280.569	1279.5617	1.32	−H
30	11.84	2′-O-Acetyl-platycodin D2	C₆₅H₁₀₄O₃₄	1428.6409	1428.6425	1427.6352	1.12	−H
31	11.94	16-Oxo-platycodin D	C₅₇H₉₀O₂₈	1222.5619	1222.5633	1221.5561	1.21	−H
32	12.01	Platycodin A	C₅₉H₉₄O₂₉	1266.5881	1266.5888	1311.587	0.55	+HCOO

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