Table 1 Primer list for the determination of gene expression level in Tetranychus urticae after ethyl formate and phosphine fumigation
Gene name | Symbol | Function | Size (bp) | Primer sequence (5′-3′) |
|---|---|---|---|---|
Ribosomal protein L13A | rpl13 | Housekeeping gene | 104 | F) GCTCACAGCCTATGAAGGTATT |
R) GAACTTACGACCTCCTTGTAATC | ||||
Glyceraldehyde-3-phosphate dehydrogenase | gapdh | Housekeeping gene | 99 | F) CGATGCGCCTATGTTTGTTATG |
R) GGAGCAAGACAGTTGGTTGTA | ||||
NADH dehydrogenase [ubiquinone] flavoprotein 1 | ndufv1 | Mitochondrial electron transport | 105 | F) CGATGAAGGAGAACCTGGCA |
R) CAGGCACCCATAGCCTTACC | ||||
Cytochrome c oxidase assembly protein COX11 | cox11 | Mitochondrial electron transport | 101 | F) TCAGTCCTGGGGAGACAACA |
R) AGCTTCGAACGGCAGAACAG | ||||
ATP synthase subunit beta | atpB | ATP synthesis | 115 | F) TTGCTGGTGTCGGAGAAAGA |
R) TGACCGTATACAAGGGCGAC | ||||
Voltage-gated sodium channel alpha subunit | para | Regulation of ion transmembrane transport | 95 | F) GCCGGTCTTGCCAAAATTCA |
R) ACCCTCATCCCTTTAGACCGA | ||||
Acetylcholinesterase-like | ace | Acetylcholine catabolic process | 104 | F) CGATGAAGGAGAACCTGGCA |
R) CAGGCACCCATAGCCTTACC | ||||
Septin-11-like | sept11 | Cell cycle and division | 107 | F) CCGGACCATTAGAGAAGGC |
R) CATCGGGAAACTTGGTGTTGA |