Table 1 Macromolecule-production information

DNA source

Genomic DNA

Forward primer^a

5′-GCG GCG CAT ATG AAA AAA GTT CTT GTC GTC-3′

Reverse primer^a

5′-CTA CTA CTC GAG CTC TTG AAG CGT TTC GGT-3′

Cloning vector

pET-28a

Expression vector

pET-28a

Expression host

E. coli BL21 (DE3)

Complete amino-acid sequence

of the construct produced^b

MGSSHHHHHHSSGLVPRGSHMKKVLVVDDEPSILTLLTFNLEKEGYQVTTSENGKNGFELALSNQYDFIILDVMLPGMDGLEITKALRREKIDTPILILTAKDEQVDKIIGLEIGADDYLTKPFSPREVLARMKAIFRRLKPTTTETLQELEHHHHHH