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Table 2 Data-collection statistics

From: Overexpression, purification, crystallization and preliminary X-ray crystallographic characterization of the receiver domain of the response regulator PhoP from Enterococcus faecalis ATCC 29212

 

EfPhoP-RD

Thick plate-shape

Thin rod-shape

Diffraction source

Beamline 7A, PAL

Wavelength (Å)

1.0000

Detector

ADSC Quantum 270r CCD

Crystal-to-detector distance (mm)

450

350

Rotation range per image (°)

1

1

Total rotation range (°)

180

360

Exposure time per image (s)

5

2

Space group

P212121

C2221

Unit-cell parameters (Å, °)

a = 188.63, b = 187.71, c = 197.49

α  =  β  =  γ  = 90

a = 118.74, b = 189.83, c = 189.88

α  =  β  =  γ  = 90

Resolution range (Å)

50.0−5.0 (5.18−5.00)

50.0−3.5 (3.63−3.50)

Total no. of reflections

628,425

1,931,024

No. of unique reflections

26,755

20,764

Completeness (%)

87.1 (81.2)

75.4 (65.1)

Multiplicity

3.0 (1.9)

2.5 (1.7)

<I/σ(I)>

10.8 (2.0)

6.6 (2.4)

CC1/2

0.994 (0.172)

0.973 (0.334)

Ramerge (%)

10.1 (35.1)

14.3 (34.9)

  1. Values in parentheses are for the outer shell
  2. aRmerge = ∑hkli|Ihkli−< Ihkl> |/∑hkli Ihkli, where I represents the observed intensity, < I > represents the average intensity, and i counts through all symmetry-related reflections