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Applied Biological Chemistry
Fig. 1 | Applied Biological Chemistry

Fig. 1

From: Production, characterization, and cross-reactivity of a polyclonal antibody against Arabidopsis TARGET OF RAPAMYCIN

Fig. 1

Design and expression of recombinant truncated Arabidopsis TOR fragments in E. coli. a Schematic representation of Arabidopsis TOR and design of truncated fragments for antigens. Two fragments of TOR, 1–200 aa (TOR1−200) and 1113–1304 aa (TOR1113−1304), are shown as bars under the TOR protein. HEAT repeats, Huntingtin, Elongation factor 3, subunit of PP2A, and TOR1; FAT, FRAP-ATM-TRRAP domain; FRB, FKP12-rapamycin binding domain; FATC: C-terminal FAT domain. b, c Expression of recombinant His-TOR1−200 (b) and TOR1113−1304 (c) proteins in E. coli. Cells harboring gRSET::TOR1−200 and gRSET::TOR1113−1304 were individually induced by 0, 0.1, and 0.5 mM IPTG in a small-scale culture (3 mL LB liquid media). Insoluble pellet (Ppt) and supernatant (Sup) fractions were separated in 10% SDS-PAGE. Molecular size markers (kDa) are represented on the left. Arrows show the recombinant His-TOR1−200 (b) and His-TOR1113−1304 (c) proteins

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