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Applied Biological Chemistry
Fig. 5 | Applied Biological Chemistry

Fig. 5

From: Production, characterization, and cross-reactivity of a polyclonal antibody against Arabidopsis TARGET OF RAPAMYCIN

Fig. 5

Immunoprecipitation assay using purified polyclonal α-TOR antibody. Proteins of Arabidopsis wild-type and tor-est seedlings, as prepared in Fig. 3a, were extracted, and the proteins (Input) were mixed with purified polyclonal α-TOR antibody-bound protein A-agarose beads (IP). Input and isolated proteins by IP were separated in 6% SDS-PAGE, and immunoblot analysis was performed against α-TOR antibody (1:500 dilution). Molecular size markers (kDa) are represented on the left

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