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Applied Biological Chemistry
Fig. 3 | Applied Biological Chemistry

Fig. 3

From: PF4V1 affects the progression of oral squamous cell carcinoma by regulating Wnt/β-catenin pathway and angiogenesis

Fig. 3

Apoptosis analyses of CAL-27 and SCC-9 cells treated with PF4V1. PI indicated propidium iodide. The quantitative analysis of positive Annexin V CAL-27 cells in different groups (a). The quantitative analysis of positive Annexin V SCC-9 cells in different groups (b). Representative scatter plots of PI (y-axis) versus Annexin V (x-axis). Upper left, lower left, upper right and lower right in the cell distribution panels represented the proportions of viable cells, dead cells, late and early apoptotic cells, respectively. The quantitative analysis of Bcl-2 and Caspase-3 in different groups in RT-PCR test (c). c, dCaspase-3 mRNA expression level in SCC-9 and CAL-27 cells. e, fBcl-2 mRNA expression level in SCC-9 and CAL-27 cells. PF4V1: PF4V1 overexpression group; Ctrl-PF4V1: the control vector of PF4V1 overexpression group; Ctrl-shRNA: the control vector of PF4V1 silence group; PF4V1-shRNA: PF4V1 silence group. *P < 0.05, ***P < 0.01, ***P < 0.001 versus the corresponding control group

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