Table 1 The primer information in RT-PCR assay
Pathway | Gene name | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|---|
Wnt/β-catenin pathway | PF4V1 | CAGAACCCCAGCCCGACTTTCCC | TTAATCAGCAGATTCATCACCTGCC |
GAPDH | CTCCTCCACCTTTGACGCTG | TCCTCTTGTGCTCTTGCTGG | |
VEGF | GCCTTGCCTTGCTGCTCTACC | GGTCTCGATTGGATGGCAGTAGC | |
GSK-3β | CAGAGACAAGGACGGCAGCAAG | GGCGACCAGTTCTCCTGAATCAC | |
FGF | GTGCTAACCGTTACCTGGCT | CCCAGTTCGTTTCAGTGCCA | |
β-catenin | GGCTCTTGTGCGTACTGTCCTTC | GCTTCTTGGTGTCGGCTGGTC | |
EMT pathway | Snail1 | GGCTCCTTCGTCCTTCTCCTCTAC | CCAGGCTGAGGTATTCCTTGTTGC |
Snail2 | CCATGCCTGTCATACCACAACCAG | GGAGGAGGTGTCAGATGGAGGAG | |
Twist | CACCATCCTCACACCTCTGCATTC | GGCTGATTGGCACGACCTCTTG | |
Apoptosis-related genes | Bcl-2 | ATCGCCCTGTGGATGACTGAG | CAGCCAGGAGAAATCAAACAGAGG |
Caspase-3 | CCTGTGGCTGTGTATCCGTG | CCGAGATGTCATTCCAGTGC |