Fig. 1

Inhibitory effect of Hispidin on LPS induced NO prodution in RAW264.7 cells. a Chemical structure of Hispidin. b Cells were treated with different concentrations of Hispidin and incubated for 24 h. Cell viability was measured by MTT assay. c, d RAW264.7 cells was pre-treated with indicated concentrations of Hispidin for 30 min followed by LPS (1 μg/ml) stimulation for 24 h. The levels of NO production was measured by Griess reagent. e, f The protein expression levels of iNOS were detected by western blot by treatment with LPS and Hispidin combined with LPS in time- and dose-dependent manner. g, h The related protein expression levels were represented with as the mean ± SD. Data are presented as the mean standard error of the mean of three different samples. *P < 0.05, **P < 0.01, and ***P < 0.001