Fig. 3

Effect of Hispidin on LPS induced ROS and phagocytosis in RAW264.7 macrophage cells. Cells were pre-treated with 20 µg/ml Hispidin for 30 min, followed by treatment with LPS (1 µg/ml) for the 24 h. The level of cellular ROS and phagocytosis were detected using (a, d) flow cytometry (DCFH-DA for ROS and Alexa 488 for phagocytosis) and (b, e) fluorescence microscopy (DHE (red) for ROS and Hochest for nuclear; Alexa 488 for phagocytosis), scale bar = 200 mm. c, f The ROS and phagocytosis were confirmed in a time-dependent durations with flow cytometry and the related data were presented as the mean ± standard error of the mean for three different samples. **P < 0.01, ***P < 0.001