Table 1 Primers used for quantitative real-time PCR analysis of tight junction-related genes
Gene |  | Primer sequence (5′ to 3′) | Product size (bp) | Annealing temp. (°C) |
|---|---|---|---|---|
ZO-1 | F R | AGA GCA CAG CAA TGG AGG AA GAC GTT TCC CCA CTC TGA AA | 133 | 58 |
Occludin | F R | TTT GTG GGA CAA GGA ACA CA ATG CCA TGG GAC TGT CAA CT | 137 | 58 |
Claudin 1 | F R | GCA GAT CCA GTG CAA AGT CT CAT ACA CTT CAT GCC AAC GG | 136 | 58 |
Claudin 4 | F R | CGC ATC AGG ACT GGC TTT AT AGT TGA GGA CCT GGA AGG CT | 131 | 58 |
Claudin 6 | F R | GGC CCT CTG AGT ACC CTA CC GCA GGA GGC AGA AAC AAA AG | 136 | 58 |
Claudin 8 | F R | GGC TGT TTC TTG GTG GTG TT CAC GCA ATT CAT CCA CAG TC | 137 | 58 |
β-Actin | F | TCA CCC ACA CTG TGC CCA TCT ACG A | 295 | 58 |
R | CAG GGG AAC CGC TCA TTG CCA ATG G |