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Applied Biological Chemistry
Fig. 1 | Applied Biological Chemistry

Fig. 1

From: Isoflavones and soyasaponins in the germ of Korean soybean [Glycine max (L.) Merr.] cultivars and their compound-enhanced BMP-2-induced bone formation

Fig. 1

Isoflavones enhance osteoblast differentiation. a C2C12 cells were cultured for 3 d with BMP-2 (100 ng/mL) with either the vehicle (DMSO) or the indicated concentration of isoflavones. Osteoblast differentiation was visualized by ALP staining. b The ALP activity was determined by measuring absorbance at 405 nm. ###p < 0.001 (versus control); ***p < 0.001 (versus BMP-2-treated group). c The effects of isoflavones on the C2C12 cell viability were evaluated by the CCK-8 assay. The data are shown as the mean ± SD and are representative of the three experiments

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