Microencapsulation of microbial antioxidants from Mucor circinelloides, their physico-chemical characterization, in vitro digestion and releasing behaviors in food

Applied Biological Chemistry

Table 3 The observations of general appearance, behavior, organ-to-body weight index, and biochemical parameters of serum for control and treated groups of albino mice

Observations	Control group^b	Treated group^c
General observations^a
Skin and fur	Normal	Normal
Eyes	Normal	Normal
Mucous membranes	Normal	Normal
Behavioral changes	Normal	Normal
Salivation	Normal	Normal
Urination (color)	Normal	Normal
Lethargy	Normal	Normal
Sleep	Normal	Normal
Diarrhea	Not found	Not found
Coma	Not found	Not found
Tremors	Not found	Not found
Organ-to-body weight index¹ (%)
Kidney	1.11 ± 0.17	1.53 ± 0.23^a
Heart	0.57 ± 0.07	1.23 ± 0.2^a
Liver	4.8 ± 0.39	6.87 ± 0.47^a
Lung	0.79 ± 0.04	1.53 ± 0.11^a
Spleen	0.67 ± 0.09	1.77 ± 0.1^a
Body weight	22.7 ± 0.8	24.7 ± 1.3^a
Biochemical parameters of serum
TBIL (µmol/L)	1.2 ± 0.2	0.9 ± 0.1
ALT (U/L)	17.7 ± 4.4	18.1 ± 5.1
AST (U/L)	81.4 ± 3.7	79.1 ± 5.2
ALP (U/L)	86.3 ± 9.6	87.7 ± 11.3

^aGeneral observations were taken through the treatment period started right after oral administration of specific doses
^bControl groups (treatment without crude extract)
^cTreated groups (treatment with 2000 mg/kg crude extract)
¹Organ body index = (organ weight × 100)/body weight; microcapsule (T_A)/crude ethanolic extracts was administered to mice at a dose of 2000 mg/kg; values are mean ± SD (n = 21) at 5% level of significance (*p < 0.05)