Table 1 Primers sets used to detect hybrids between non-transgenic soybean and transgenic soybean events (CT-1001, CT-2062, and CT-4025 with egf, igf-1, and trx genes, respectively)
Target gene | Primer | Sequence (5′ to 3′) | Amplicon size (bp) |
|---|---|---|---|
egf | EGF-F | CAC TTT CCC ACG ATG GAT ACT G | 143 |
EGF-R | TTA CCT AAG CTC CCA CCA CTT | ||
igf-1 | IGF-F | GAG CTT GTT GAT GCT CTC CAA T | 114 |
IGF-R | CTC GTC CAC AAT TCC AGT TTG T | ||
trx | TRX-F | GCA AGA TGA TCA AGC CCT TCT T | 185 |
TRX-R | CTT CTC TTT GTT AGC ACC GGA G | ||
lectin | Lectin-F | ATC CGA CGA TGG ATT TCT TG | 217 |
Lectin-R | GGC GGC ATT ATA GGT AAC GA |