Fig. 1

Effects of viscothionin on AMPK signal pathway in 3T3-L1 cells. Cells were pre-treated with compound C for 1 h, and then treated with 5 μM viscothionin for 8 days. On day 8, completely differentiated cells were lysed to extract total protein. a Protein extracts were prepared and subjected to Western blot analysis using p-AMPK, AMPK, p-ACC, ACC, p-AKT, AKT, p-GSK3β, GSK3β, and β-actin antibodies. Each total protein level was used as an internal control to elevate relative expression of the phosphorylated protein. b–e Quantitative analysis of Western blot in a. Data are represented as mean ± SEM of three different preparations. **p < 0.01, and ***p < 0.001, as compared with control. ^##p < 0.01, and ^###p < 0.001, as compared with only DMI. ⁺p < 0.05,  ⁺⁺p < 0.01, and ⁺⁺⁺p < 0.001, as compared with DMI + viscothionin 5 μM