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Applied Biological Chemistry
Fig. 4 | Applied Biological Chemistry

Fig. 4

From: A trienone analog of curcumin, 1,7-bis(3-hydroxyphenyl)-1,4,6-heptatrien-3-one, possesses ROS- and caspase-mediated apoptosis in human oral squamous cell carcinoma cells in vitro

Fig. 4

The effects of trienone 11 on the activation of caspase-3/7, caspase-8, and caspase-9 in CLS-354/DX cells. The cells were treated with trienone 11 (30 μM) for 6 h. After treatment, protein lysates (100 μg protein) were incubated with the substrates of caspase-3/7, caspase-8, and caspase-9 for indicated times at 37 °C for 3 and 24 h. Activities of caspase-3/7 (a), caspase-8 (b), and caspase-9 (c) were measured by a microplate reader. Values are expressed as mean ± standard deviation (SD) from four independent treatments. Different letters above the bar indicate the significant difference (p < 0.05)

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