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Applied Biological Chemistry
Fig. 5 | Applied Biological Chemistry

Fig. 5

From: A trienone analog of curcumin, 1,7-bis(3-hydroxyphenyl)-1,4,6-heptatrien-3-one, possesses ROS- and caspase-mediated apoptosis in human oral squamous cell carcinoma cells in vitro

Fig. 5

The effects of trienone 11 on production of reactive oxygen species (ROS) in CLS-354/DX cells. CLS-354/DX cells were treated with 15–30 μM trienone 11 for 12 h with/without 5 mM N-acetyl cysteine (NAC) pre-treatment. Intracellular ROS was detected using H2DCFDA probe followed by flow cytometric analysis. ROS levels are represented in a histogram (a) and mean ± standard deviation (SD) of fluorescent intensities (MFI) (b). Values are expressed as mean ± standard deviation (SD) of duplicate samples in three independent experiments. Different letters above the bar indicate the significant difference (p < 0.05)

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