Simultaneous determination of 13 mycotoxins in feedstuffs using QuEChERS extraction

Applied Biological Chemistry

Table 1 Optimized LC–MS/MS parameters for the determination of mycotoxins in feedstuffs

Mycotoxin	Retention Time (min)	Ionization	Precursor ion (m/z)	Product Ion (m/z)	Fragment voltage (V)	Collision energy (V)	Cell accelerator voltage (V)
Aflatoxin B₁	4.53	[M+H]⁺	313.0	285.0	175	25	5
Aflatoxin B₁	4.53	[M+H]⁺	313.0	241.0	175	40	5
Aflatoxin B₂	4.42	[M+H]⁺	315.0	259.0	185	35	5
Aflatoxin B₂	4.42	[M+H]⁺	315.0	287.0	185	35	5
Aflatoxin G₁	4.29	[M+H]⁺	329.0	243.0	130	30	5
Aflatoxin G₁	4.29	[M+H]⁺	329.0	311.0	130	20	5
Aflatoxin G₂	4.16	[M+H]⁺	330.9	313.0	160	25	5
Aflatoxin G₂	4.16	[M+H]⁺	330.9	245.0	160	35	5
Deoxynivalenol	3.04	[M+H]⁺	297.3	249.1	109	10	5
Deoxynivalenol	3.04	[M+H]⁺	297.3	191.0	109	58	5
Fumonisin B₁	5.08	[M+H]⁺	722.0	352.3	180	45	5
Fumonisin B₁	5.08	[M+H]⁺	722.0	704.2	180	40	5
Fumonisin B₂	5.53	[M+H]⁺	706.4	335.9	180	35	5
Fumonisin B₂	5.53	[M+H]⁺	706.4	354.3	180	30	5
Ochratoxin A	5.49	[M+H]⁺	404.1	238.9	100	25	5
Ochratoxin A	5.49	[M+H]⁺	404.1	192.7	100	50	5
T-2 toxin	5.31	[M+NH₄]⁺	483.8	215.0	140	20	5
T-2 toxin	5.31	[M+NH₄]⁺	483.8	263.1	140	10	5
HT-2 toxin	5.05	[M+NH₄]⁺	442.5	263.1	120	10	5
HT-2 toxin	5.05	[M+NH₄]⁺	442.5	215.1	120	10	5
Zearalenone	5.55	[M−H]⁻	317.0	175.1	120	25	5
Zearalenone	5.55	[M−H]⁻	317.0	186.9	120	15	5
α-zearalenol	5.38	[M−H]⁻	319.0	275.2	145	25	5
α-zearalenol	5.38	[M−H]⁻	319.0	301.1	145	25	5
β-zearalenol	5.16	[M−H]⁻	319.0	275.2	145	25	5
β-zearalenol	5.16	[M−H]⁻	319.0	301.1	145	25	5