Table 4 Recovery (n = 5) of mycotoxins obtained from QuEChRES sample preparation
From: Simultaneous determination of 13 mycotoxins in feedstuffs using QuEChERS extraction
Mycotoxin | Fortification level (µg/kg) | Recovery (%) | |||||
|---|---|---|---|---|---|---|---|
Maize | Bean | ||||||
Aver | STDEV | %RSD | Aver | STDEV | %RSD | ||
Aflatoxin B1 | 5 | 83.7 | 2.1 | 2.5 | 83.7 | 3.2 | 3.8 |
10 | 86.3 | 2.5 | 2.9 | 78.8 | 1.5 | 1.9 | |
50 | 86.3 | 3.3 | 3.8 | 85.5 | 1.0 | 1.2 | |
Aflatoxin B2 | 5 | 77.0 | 5.2 | 6.8 | 85.3 | 3.3 | 3.9 |
10 | 81.5 | 1.1 | 1.3 | 87.0 | 0.7 | 0.8 | |
50 | 83.7 | 1.9 | 2.2 | 86.6 | 0.7 | 0.9 | |
Aflatoxin G1 | 5 | 71.0 | 2.4 | 3.4 | 77.8 | 2.0 | 2.5 |
10 | 79.9 | 0.6 | 0.8 | 85.4 | 1.2 | 1.4 | |
50 | 86.1 | 1.8 | 2.1 | 87.2 | 0.1 | 0.2 | |
Aflatoxin G2 | 5 | 87.4 | 6.2 | 7.1 | 82.8 | 1.0 | 1.3 |
10 | 88.9 | 3.0 | 3.3 | 88.8 | 2.1 | 2.4 | |
50 | 88.4 | 2.0 | 2.3 | 83.1 | 0.9 | 1.1 | |
Deoxynivalenol | 100 | 89.0 | 10.0 | 11.3 | 100.5 | 2.1 | 2.1 |
200 | 76.7 | 2.7 | 3.5 | 87.0 | 3.4 | 3.9 | |
1000 | 78.5 | 5.5 | 6.9 | 74.9 | 3.9 | 5.2 | |
Fumonisin B1 | 100 | 99.0 | 3.3 | 3.4 | 86.7 | 3.2 | 3.6 |
200 | 73.9 | 1.3 | 1.8 | 78.1 | 2.2 | 1.5 | |
1000 | 75.0 | 1.1 | 1.4 | 72.1 | 1.1 | 1.5 | |
Fumonisin B2 | 100 | 112.3 | 2.9 | 2.6 | 115.6 | 4.1 | 3.5 |
200 | 80.9 | 7.5 | 9.3 | 88.0 | 2.6 | 2.9 | |
1000 | 73.3 | 1.5 | 2.0 | 73.1 | 1.5 | 2.0 | |
Ochratoxin A | 20 | 106.3 | 1.8 | 1.7 | 96.4 | 1.8 | 1.9 |
40 | 76.6 | 1.3 | 1.7 | 89.3 | 1.7 | 1.8 | |
200 | 88.1 | 0.6 | 0.7 | 82.5 | 1.0 | 1.2 | |
T-2 toxin | 100 | 86.7 | 2.9 | 3.3 | 89.3 | 2.3 | 2.6 |
200 | 90.0 | 1.2 | 1.3 | 94.7 | 1.2 | 1.3 | |
1000 | 88.7 | 1.6 | 1.8 | 93.7 | 1.9 | 2.0 | |
HT-2 toxin | 100 | 72.8 | 3.2 | 4.4 | 86.5 | 2.96 | 3.1 |
200 | 83.2 | 1.7 | 2.0 | 92.2 | 3.9 | 4.2 | |
1000 | 89.6 | 2.4 | 2.7 | 90.2 | 3.9 | 1.2 | |
Zeralenone | 25 | 70.1 | 4.2 | 5.9 | 88.5 | 2.8 | 3.2 |
50 | 83.3 | 3.2 | 3.8 | 84.1 | 3.6 | 4.3 | |
100 | 89.4 | 1.9 | 2.1 | 80.5 | 1.9 | 2.4 | |
α-zearalenol | 100 | 75.2 | 3.4 | 4.5 | 111.6 | 2.7 | 2.4 |
200 | 82.8 | 2.5 | 3.0 | 97.8 | 7.0 | 7.1 | |
1000 | 86.3 | 3.3 | 3.8 | 88.7 | 2.0 | 2.2 | |
β-zearalenol | 100 | 94.1 | 6.0 | 6.4 | 83.6 | 2.5 | 3.0 |
200 | 86.3 | 1.0 | 1.1 | 84.0 | 3.5 | 4.1 | |
1000 | 84.4 | 3.4 | 4.0 | 84.4 | 1.7 | 2.0 | |