Determination of antioxidant activity and phenolic compounds for basic standardization of Turkish propolis

Applied Biological Chemistry

Table 2 Validation parameters of HPLC

Compound	R²	Recovery (%)	LOD (mg/kg)	LOQ (mg/kg)	5 mg/L		20 mg/L		40 mg/L
					RSR_r	RSD_R	RSR_r	RSD_R	RSR_r	RSD_R
Quercetin	0.9975	87.9	1.14	1.51	0.66	1.89	1.57	1.93	1.81	3.09
Galangin	0.9936	80.0	0.93	1.58	0.72	1.51	0.78	1.67	1.09	1.81
Apigenin	0.9987	104.9	1.13	1.90	0.75	1.01	1.05	1.26	1.16	1.93
Pinocembrin	0.9969	110.2	0.70	1.20	0.78	1.51	1.75	2.27	1.98	2.30
Caffeic acid	0.9983	108.4	0.89	1.45	0.30	1.52	1.22	2.23	1.31	2.93
p-Coumaric acid	0.9980	106.2	0.67	0.93	0.23	1.22	1.32	1.47	1.43	1.68
Trans-ferulic acid	0.9983	98.7	0.56	0.60	0.37	1.12	1.56	1.82	1.78	2.21
Protocatechuic acid	0.9983	108.7	0.51	0.82	0.80	1.18	0.97	1.24	1.07	1.92
Trans-cinnamic acid	0.9984	88.5	0.61	0.66	0.57	1.06	0.91	1.44	1.09	1.76
Caffeic acid Phenethyl ester (CAPE)	0.9983	98.9	0.51	1.18	0.24	1.37	1.45	1.83	1.59	2.37