Selection of the high efficient sgRNA for CRISPR-Cas9 to edit herbicide related genes, PDS, ALS, and EPSPS in tomato

Applied Biological Chemistry

Table 1 Selected sgRNA for targeting herbicide resistance genes

Gene full name	Target gene name	sgRNA sequence	On-score*
Phytoene desaturase1	PDS_c	TTAACTGTATTGTCCAGCTC	0.2317
Phytoene desaturase2	PDS_R	CGCATTGAACAGGTTTCTTC	0.0187
Acetolactate synthase 1	ALS1_W	ATTTGGGAATGGCGACACAG	0.3573
Acetolactate synthase 2	ALS2_W	ACTTGGGAATGGTGGTTCAA	0.0626
Acetolactate synthase 2	ALS2_P	CTATTACAGGTCAAGTGCCA	0.2895
5-Enolpyruvylshikimate-3-phosphate synthase	EPSPS_GT	TTGCTGTTCCTGCATTTCCA	0.3950
	EPSPS_c1	TCTGGTACTGTTAAATTACC	0.4788
	EPSPS_c2	CTGTAGCTGGAGGACATTCA	0.0776

^*sgRNA target score estimated using the CRISPR-P 2.0 program. http://crispr.hzau.edu.cn/CRISPR2