Selection of the high efficient sgRNA for CRISPR-Cas9 to edit herbicide related genes, PDS, ALS, and EPSPS in tomato

Yang, So Hee; Kim, Euyeon; Park, Hyosun; Koo, Yeonjong

doi:10.1186/s13765-022-00679-w

Applied Biological Chemistry

Table 2 Primer sets for amplifying target genes

From: Selection of the high efficient sgRNA for CRISPR-Cas9 to edit herbicide related genes, PDS, ALS, and EPSPS in tomato

Target gene	Primer		Sequence
PDS_c	1st primer	Forward	GAGCTCGAGGTCGTCTTCTT
	1st primer	Reverse	TGCTCCTAGTCCAATCAGCAG
	2nd primer	Forward	ACACTCTTTCCCTACACGACGCTCTTCCGATCTTGGTTAAGGACTTGGGGCCT
	2nd primer	Reverse	GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTTTTAGTTGGGCGCGGAGAAG
PDS_R	1st primer	Forward	TGTTAAGGACTGGATGAGAAAGCA
	1st primer	Reverse	ATATGCCCTCAACCTAGAGAAACC
	2nd primer	Forward	ACACTCTTTCCCTACACGACGCTCTTCCGATCTATTGCTATGTCAAAGGCACTCAAC
	2nd primer	Reverse	GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTATGTTATGTGAGAGTCTGGAAGGG
ALS1_W	1st primer	Forward	TGTGGCGAGTACTTTGATGGGACT
	1st primer	Reverse	GCACCATTACTGGGAATCATAGGC
	2nd primer	Forward	ACACTCTTTCCCTACACGACGCTCTTCCGATCTGTAGACATCGATGGGGATGGTAGT
	2nd primer	Reverse	GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTCACAAGCCTCCGCGAATTTCAACA
ALS2_W	1st primer	Forward	TCCAACTGGGGATGAGCTTTCACT
	1st primer	Reverse	GCCCTCCGTGATCACATCTTTGAA
	2nd primer	Forward	ACACTCTTTCCCTACACGACGCTCTTCCGATCTGTTGACATTGACGGTGATGGGAGT
	2nd primer	Reverse	GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGAAGGGTCACCCAAGTAAGTGTGT
ALS2_P	1st primer	Forward	CCCATAACGACGTTTCTGAACCTG
	1st primer	Reverse	CCCTCCAATATGGAATTCAAACCC
	2nd primer	Forward	ACACTCTTTCCCTACACGACGCTCTTCCGATCTGGTGTTTGCATTGCTACCTCTGGT
	2nd primer	Reverse	GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGCTTCACGAACAACCCTAGGAATA
EPSPS_GT	1st primer	Forward	TGACGACATTCATTACATGCTTGG
	1st primer	Reverse	ATGCTTTCCCAGGAGATTTGTACT
	2nd primer	Forward	ACACTCTTTCCCTACACGACGCTCTTCCGATCTTGACAATGAAAACCAACGAGCAAT
	2nd primer	Reverse	GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGGTCTCTCTCTCATCCTAGGAACT
EPSPS_c1	1st primer	Forward	AAGGGATACAGACCCTTAGTCTGA
	1st primer	Reverse	GCTGCTTAAGACCATCAACCAAAT
	2nd primer	Forward	ACACTCTTTCCCTACACGACGCTCTTCCGATCTTTTTAGGATTTCTGCATCAGTGGC
	2nd primer	Reverse	GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTAAATTGTCAACAACAGTCCTTCCC
EPSPS_c2	1st primer	Forward	TGACGACATTCATTACATGCTTGG
	1st primer	Reverse	ATGCTTTCCCAGGAGATTTGTACT
	2nd primer	Forward	ACACTCTTTCCCTACACGACGCTCTTCCGATCTTGTCGGTAAAAAGTCTGAGGAAGA
	2nd primer	Reverse	GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGCTGCTTAAGACCATCAACCAAAT

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