Fig. 3

SM is an agonist of NRF2 complex and CD4 mediators, A, B immunoprecipitation of NRF2 with ARE complex in SM treated CD3 activated CD4 cells were blotted using immuno blot. B mRNA expression of NRF2 was quantified using quantitative real time PCR and C SM modulates the nuclear oxidative protein and quantified using Immunoblot of NRF2 protein and Tubulin used as internal control. D Immunohistological score of NRF2 in SM treated EAE mice and naïve mice were evaluated and presented in NRF2 expression score. All data were expressed as mean ± SEM and the differences between variables were analyzed using student T test. The differences were considered significant when the P values were less than 0.05