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Fig. 6 | Applied Biological Chemistry

Fig. 6

From: Glycyrrhiza uralensis attenuates TNF-α-induced muscle atrophy in myoblast cells through the Nrf2 and MAFbx signaling cascades

Fig. 6

Protective effect of Glycyrrhiza uralensis on tumor necrosis factor (TNF)-α-induced C2C12 cell death. C2C12 cells were plated onto 6-well plates at a density of 5 × 105 cells/well. After treatment with different concentrations (10, 50 and 100 μg/mL) of GU or 10 ng/mL of TNF-α for 12 or 24 h, the expression levels of cleaved caspase-3, -7, phosphoinositide 3-kinases (PI3K), phospho (p)-PI3K, protein kinase B (Akt), and p-Akt proteins were measured using western blot analysis (A). Each protein expression was normalized to total expression of β-actin and total form of PI3K and Akt. Caspase-3/7 activity was quantified by using MUSE analysis (B). Data are presented as the means ± standard error of the means (SEMs) of three independent experiments

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