Fig. 2
From: Caenorhabditis elegans as a powerful tool in natural product bioactivity research
Working scheme of HTS for screening natural products (NPs) that improve gut permeability. Age synchronized worms are infected with the pathogen and cotreated with NPs for an appropriate time. Worms are then fed with FITC-dextran as an indicator of gut permeability. After washing and fixing in 96-well plates, a high-throughput Operetta machine is used to measure the intestinal permeability of C. elegans. Infected worms show higher fluorescence intensity due to intestinal damage. Effective compounds are screened as having lower fluorescence intensity, indicating the improving effect against gut barrier dysfunction